Research |
"There is nothing permanent except change"
Heraclitus - 540-480 BC
© Copyright 1995- 2000 Colleen M. Allen
Last update May 2000
(by Michael Roussin
© Copyright 1996 Michael Roussin , All rights reserved)
This material has been removed at the authors request. He is editing the material and will place it on line as a E book. The material removed is the newest and most accurate to date about kombucha and its analysis. Mike was kind enough to allow us to present the material for about 5 years. He is trying to recoup the costs incurred by the research group while they were doing the analysis. I wish him luck and fully support him. His current website follows:
http://www.kombucha-research.com
Bob Williams / Current owner of the Kombucha FAQ
This information is reprinted with permission from John Novar of
Kombucha Power.
The following lab.analysis was done by Kappa Labs in Miami Florida.
Serving Size | 4 fluid ounces (120 ml) |
Servings Per Container | 8 |
Calories Per Serving | 40 |
Calories per Serving From Fat | 0 |
PERCENT DAILY REQUIREMENTS |
|
Total Fat | 0g - 0% |
Sodium | 0mg - 0% |
Total Carbohydrate | 8g - 4% |
Sugar | 8g - 4% |
Protein | 0g - 0% |
VITAMIN CONTENT |
|
Vitamin C | 0.1152 mg |
Niacinamide | 0.6420 mg |
Folic Acid | 0.2794 mg |
Riboflavin | 1.1594 mg |
Sucrose | 0.22 g |
Glucose | 0.78 g |
Fructose | 0.65 g |
Total Amount Sugar | 1.65 g |
John M. Novar
13055 SW 83rd Avenue
Pinecrest, FL 33156-6648
http://www.kombuchapower.com/
800-862-1353 - 305-251-9630 - Fax 360-397-7080
"John Novar" <Mailto:jnovar@netzero.net>
KAPPA LABORATORIES, INC.
2577 N.W. 74th Avenue, MIAMI, Florida 33122
Phone (305) 599-0199 FAX (305)592-1224
May 7, 1996
CERTIFICATE OF ANALYSIS
Kombucha Power Products
2121 Ponce De Leon Blvd.
Suite 522 Coral Gables, Florida 33134
Re: Microbiological results obtained from
one (1) Kombucha Tea, bottled 12/95.
The sample was delivered to Kappa Laboratories, Inc., 2577 N.W. 74th Avenue, Miami, Florida, 33122 on April 17, 1996. The product was submitted by Kombucha Power Products, 2121 Ponce De Leon, Suite 522, Coral Gables, Florida 33134
Microbiological sampling and preparation of one (1) specimen was
carried out according to the AOAC Methods 46.013. The following tests listed were performed according to the AOAC
methods cited:
Coaqulase-Positive Staphylococcus (Staphylococcus aureus): was assayed
according to AOAC methods 46.136, 14th ed. to determine count/gm sample using direct triplicate plating on Baird-Parker
agar with direct colony count. Positive colonies were confirmed by the Coaqulase reaction.KAPPA LABORATORIES, INC.
Total Plate Count | <100 cts. per gram |
Coliforms | <3.0 cts. per gram |
E.coli | <3.0 cts. per gram |
Coaqulase Positive Staph. Aureus | <100 cts. per gram |
Total Plate Count | Negative |
Coliforms | Negative |
E.coli | Negative |
Coagulase Positive Staph. Aureus | Negative |
Note: less than (<) sign indicates Negative growth for that bacteria at the lowest level expressed. For example, <10 cts. Per gram would indicate Negative, or no growth, for the bacteria at a concentration of 1.10, if the bacteria is present it is less than this concentration.
Based upon the Microbiological Results of the samples listed above and insofar as the analyses performed were able to assay for the presence or absence of specific pathogens, the product represented by the sample analyzed would appear to be wholesome and fit for human consumption.
Kappa Laboratories has been inspected and is currently certified by the U.S. Department of Agriculture (USDA Microbiology-#0093): the Florida Dept. of Health and Rehabilitative Services, in Microbiology (HRS-#86260); Pesticides, Herbicides, PCB's (HPLC) (HRS (E86515): Registered with the U.S. Food and Drug Administration (FDA-#1039389) and is an FDA Accepted Laboratory for Import Testing. Kappa Laboratories is an elected Associate Member of the Meat Importers Council of America, Inc. (MICA).
Signed: Dr. Peter J. Kmieck-Director, Kappa Inc.
Copyright@ 1996 Kombucha Power Products
John M. Novar
13055 SW 83rd Avenue
Pinecrest, FL 33156-6648
http://www.kombuchapower.com/
800-862-1353 - 305-251-9630 - Fax 360-397-7080
"John Novar" <Mailto:jnovar@netzero.net>
All rights reserved. No part of this certificate shall be reproduced, stored in a retrieval system, or transmitted by any means, electronic, mechanical, photocopying, recording, or otherwise without written permission from the publisher.
Département de Génie Biochimique et Alimentaire, UA CNRS N°544, Institut National des Sciences Appliquées, Complexe Scientifique de Rangueil F-31077 Toulouse cedex
This article is reprinted with permission from Dr. Blanc.
This symbiotic culture is grown traditionally on black tea with sucrose for 7 days and gives a pleasantly sour and sparkling beverage (teakwass) under aerobic conditions (Reiss, 1994). Longer incubations results in an increasing production of acetic acid and in the formation of a mild vinegar. The consumption of fermented tea was first practiced in 220 BC. in Manchuria. It then spread to Russia where a wide literature on teakwas is available (Frank, 1990). During W.W.II, this beverage was introduced to Germany, then in the 50's, it arrived in France as well as France-dominated North Africa, where its consumption was quite popular (Chambionnat, 1952; Zottner, 1952; Abadie, 1961). Presently, its consumption is popular in the United States, this popularity is mainly due to its refreshing power (because of its low ethanol content) and to speculative curative effects (including detoxification properties from the high content in glucuronic acid, presence of vitamins B1, B2 and B6, antibacterial properties due to the presence of usnic acid (Steiger and Steineger, 1957; Stadelman, 1961; Hauser, 1990).
A similar cellulose network floating at the surface of various fruit juices (namely coconut and pineapple) fermented by a symbiotic culture composed of Acetobacter xylinum and yeasts and named "Nata" is consumed in the Philippines as a delicacy (Lapuz et al., 1967; Dolendo and Maniquis, 1967). Lastly, in Brazil, this cellulose network is used for the treatment of burns and other dermal injuries and is produced by a pure culture of Acetobacter xylinum grown on a medium composed mainly of sucrose and tea xanthines (Fontana et al., 1991).
This work will focus mainly on the presence of ethanol, organic and usnic acids in fermented tea.
SUCROSE INITIAL CONCENTRATION |
||||
Day of incubation |
0 |
50 g/l |
70 g/l |
100 g/l |
0 |
7 |
7 |
7 |
7 |
1 |
4 |
4 |
4 |
4 |
3 |
3 |
3.5 |
3.5 |
3.5 |
5 |
3 |
2.5 |
2.5 |
2.5 |
6 |
3 |
2.5 |
2.5 |
2.5 |
10 |
3 |
3 |
2.5 |
2 |
12 |
- |
2 |
2 |
2 |
In the four flasks, ethanol concentration rose to a maximum and subsequently declined. The maximal concentration (1.34 g/l) was obtained after 5 days of incubation in the flask with 100 g/l initial concentration of sucrose.
The acetic acid which was converted from ethanol rose also to a maximum (4.5-5.6 g/l) until the 15 days of incubation and also subsequently declined.
The gluconic acid concentration was steadily increased, reaching
high concentrations (> 30 g/l) after 20 days of incubation for 70 and 100 g/l sucrose initial concentration.
The production of lactic acid is not shown in these figures because the concentration was never higher than 0.6
g/l (sucros initial concentration 100 g/l). The presence of glucuronic acid was also detected but at very low concentrations
(<10 mg/l).
Presently, usnic acid was not detected but we intend to study tea
fungus from other origin (Europe). To go on maintaining the non-contamination of the medium after inoculation but
also to better approach the home-made conditions of culture, we intend to replace the Roux flasks by Fernbach flasks
which will allow to study higher volumes of culture with great surface of exchange with air.
Anonymous. 1983. Tea fungus. In Handbook of indigenous fermented food, K.H. Steinkraus ed. pp. 421. Marcel Dekker Inc., New York.
Chambionnat, M. (1952) Contribution à l'étude du champignon japonais. Bull. Soc. Hist. Nat. Maroc. 22, 3-8.
Doledo, A.L. and Maniquis, P.L. 1967. Preparation and storage qualities of fortified nata de coco. The PhilippinesJ. Science. 96, 4, 363-376.
Fontana, J.D., Franco, V.C., De Souza, S.J., Lyra, I.N. and De Souza A.M. 1991. Nature of plant stimulators in the production of Acetobacter xylinum ("tea fungus") biofilm used in skin therapy. Appl. Biochem. Biotechnol. 28, 341-351.
Frank, G. 1990. Kombucha, la boisson au champignon de longue vie, W. Ennsthaler ed. Steyr, Austria.
Hauser, S.P. 1990. Dr. Sklenar's kombucha mushroom infusion-a biological cancer therapy. Schweiz Rundsch. Med. Prax. 79, 243-246.
Hesseltine, C.W. 1965. A millenium of fungi, food, and fermentation. Mycologia. 57, 2, 149-197.
Kozaki, M., Koizumi, A. and Kotahara, K. 1972. Microorganisms of zoogloeal mats formed in tea decoction. J. Food Hyg. Society (Japan). 13, 1, 89-97.
Lapuz, M.M., Galardo, E.G. and Palo M.A. 1967. The nata organism -cultural requirements, characteristics and identity. The Philippines J. Science. 96, 2, 91-109.
Reiss, J. 1989. Influence of different sugars on the metabolism of the tea. Z. Lebensm. Unters. Forsch. 198, 258-261.
Stadelman, E. 1961. Der Teepilz und seine antibiotische Wirkung. Zentralbl. Bakt. Parasit. Inf. Hyg. 180, 5, 401-435.
Steiger, K.E. and Steinegger, E. 1957. On the tea fungus. Pharmaceutica Acta Helvetiae. 32, 4, 88-93.
Zottner, G. ( 1952) Note de bactériologie à propos du champignon japonais. Bull. Soc. Hist. Nat. Maroc. 33, 9-11.
The chemist entrusted to Waldeck that he always keeps this "Russian secret home remedy" at hand, it being said that it is good for all kinds of ailments and because it naturally formed acids, it successfully counters aging problems, thus contributing to life extension.
Simultaneously, Professor Rudolf Kobert released his findings that showed he had few doubts that the beverage was highly effective against joint rheumatism.
Arauner adds to this, "that not only professors, doctors and biologists have confirmed the surprising healing successes but also those who have imbibed the mushroom-tea report about entirely excellent effects on the general body functions."
In 1944, Hans Irion, the Director of the Academy for Chemists in Braunschweig reports in the "Course for Druggist Specialty Schools (vol. 2, page 405)" that "Teekwass invigorates the entire glandular system and is highly recommended for gout and rheumatism, furunculosis, arteriosclerosis, high blood pressure and aging problems. By further harmonizing and balancing the metabolism, unwanted fat deposits in the body are removed or prevented from building up. Damaging deposits of uric acid and cholesterol are converted into more soluble forms where the body can easily excrete them via the kidneys and intestines."
Scientific exploration of the fungus commenced in the 1950s with the Moscow Bacteriological Institute (as part of their research project on the investigation of cancer throughout the country).They discovered that it was not as initially thought, a single organism but a symbiotic colony of several bacteria and yeasts with highly complex and sophisticated metabolic pathways. They isolated the following organisms: Bacterium xylinum, Bacterium xylinoides, Bacterium gluconicum, Saccharomyces ludwigii, Saccharomyces apiculatus varieties, Schizosaccharomyces pombe, Acetobacter ketogenum, Torula varieties, Pichia fermantans and other yeasts. This group of organisms shows a distinct antibiotic effect through the presence of usnic acid which is present in some lichens. There is also evidence that usnic acid may deactivate certain groups of viruses
1951 Central Oncological Research Unit and the Russian Academy of Sciences in Moscow
In 1951, the "Central Oncological Research Unit" and the "Russian Academy of Sciences in Moscow" statistically analyzed the incidence of cancer throughout all the cities and districts of the USSR. It follows that any particular area with a low incidence of cancer deserved special scrutiny from the research teams. In the research program, data was collated on environmental conditions and the everyday habits of the people were closely examined for any possible clues about their extremely high resistance to cancer.
In particular, the districts of Ssolikamsk and Beresniki - in the Western Ural Mountains - were studied since there were hardly any recorded cases of cancer. Most surprising to the researchers was that this area, in particular, was a rapidly expanding industrial zone where highly toxic metals were manufactured such as potassium, lead and mercury together with asbestos.
All this industrial pollution rated the area as one of the most toxic throughout the USSR and this was evidenced by dead fish floating in the Kama river and many species of trees were withering away with the uptake of so many industrial pollutants. Research teams focused on Ssolikamsk and Beresniki districts in an attempt to understand the unusually low incidence of cancerous disease in the population despite the surrounding environment.
Living conditions, sleeping, eating and drinking patterns were investigated. Ethnic differences were taken note of together with genetic origins of all the inhabitants. Age was also considered and despite the thorough analysis of numerous factors, the research teams lead by Dr. Molodyev and Dr. Grigoriev were unable to discern any appreciable differences to other districts in the USSR that displayed a higher cancer rate which was closer to the national average.
The mystery finally unfolded one day when Dr. Molodyev visited a local household to make more detailed inspections. On questioning the housewife about what they eat and drink he was introduced to some stoneware jars as being the source of their "Tea Kwass" a very popular drink throughout the district and one that every household enjoys. She uncovered the jars to reveal a large fungus floating on top of some liquid. To the locals, this was "Tea Kwass."
On questioning where she had obtained the fungus from, the woman said "that travelers had brought it with them from China many centuries ago. It was thought that the Chinese had originally obtained it from the Japanese." To further substantiate the possibility that there may be some connection between drinking "Tea Kwass" and a very low cancer rate, Dr. Grigoriev in Beresniki also found that every household in the region possessed the fungus and drank large quantities of the ferment each day.
In a small publication of 8 pages, titled "Cancer Diagnosis Based on Blood and the Treatment of Cancer, Pre-cancerous Conditions, and other Metabolic Diseases with Kombucha and Coli-Preparations," Dr. Sklenar reports that "vitamins, lactic acids and glucuronic acid are (viruses, bacteria, fungi) and to the dissolution of waste matter and toxic deposits (uric acid, cholesterol, etc.,) Kombucha effects an outstanding detoxification of the organism.Through enjoying this beverage there is, additionally, a noticeable invigoration of the entire glandular system and enhancement of the metabolism." Dr. Sklenar reports "that he was able to treat successfully with the mushroom-tea: gout, rheumatism, arteriosclerosis, arthritis, dysbacteria, constipation, impotence, nonspecific draining, obesity, furunculosis, kidney stones, cholesterol, cancer and especially its early stages, etc."
The information compiled for "Past Research on Kombucha Tea" was reported in the following articles:
1. An article on Kombucha by James Roche
2. Fascination of Kombucha by Guenther W. Frank.
3. Kombucha, by Tom Valentine.
The past 29 th. of November 1998, in Guadalajara Jalisco, México,
took place the 3rd. Congress of Alternative Medicine and Acupuncture, where the results of the investigation with
Magico Kombucha ® and Kombuchion ® in patients with HIV was given away.
The outcome of the study was remarkable, considering that in a period of 6 months the patients increased the CD4 cells count (between 200-500 cell/ uL ). Even though the CD8 remain high, the improvement in the clinical condition was outstanding; referring to:
Weight gain (8 to 16 pounds)
Increase of physical condition ( 20 to 30% )
Appetite increase and the remission of the following symptoms:
It would be of value to mention that during the treatment with 1 gram
of Magico Kombucha ® or Kombuchion ®, opportunistic infections didn't take place.
Both products contain Fresh freeze dried Kombucha tea capsulated in real vegetable Capsules, Vcaps ®
Yoram Azrad
Mágico Kombucha ® "El
Gran Favor Para Tu Cuerpo"
En cápsulas vcaps ® " Secreto De Una
Vida Saludable" http://www.ayoram.com
Mexico, D.F. Telefaxes (52 5) 522-5004 522-4326
542-0673
elguero@planet.com.mx (Yoram)
Included with permission from the Author, Yoram Azrad.
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Colleen M. Allen
Copyright 1996 - 2000 Colleen M. Allen
Maintained by: Bob Williams : bobwms at trib.com
UpDt: 07/07/03